Publications
2025
Super-Resolution Imaging Reveals Stretch-Induced Architectural Rearrangement of Desmoplakin in Desmosomes
Leslie D. Seeley, Collin M. Ainslie, Mary Kathryn Sewell-Loftin, and Alexa L. Mattheyses
BioRxiv
Manuscript Link
The role of actin dynamics in vesicle formation during clathrin mediated endocytosis
Jie Yuan, Yen T. B. Tran, Tomasz J. Nawara, and Alexa L. Mattheyses
BioRxiv
Manuscript Link
Desmoplakin tail domain position in the desmosomal plaque is isoform dependent
Collin M Ainslie, Krishna Patel*, Yen T. B. Tran, Navaneetha Krishnan Bharathan, Volker Spindler, and Alexa L Mattheyses
Journal of Cell Science
Manuscript Link
Research Highlight Link
First Person: Collin Ainslie and Krishna Patel Link
Cover Image Link
2024
Illuminating cellular architecture and dynamics with fluorescence polarization microscopy
Dean WF, Mattheyses AL
Journal of Cell Science
Manuscript Link
The Desmosome Comes into Focus
Bharathan NK, Mattheyses AL, and Kowalczyk AP
Journal of Cell Biology
Manuscript Link
OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images
Dean WF, Nawara TJ, Alberts RM, and Mattheyses AL
PLoS Computational Biology
Manuscript Link
Dsg2 ectodomain organization increases throughout desmosome assembly
Dean WF*, Albert RM*, Nawara TJ, Ubil M, Beggs RR, and Mattheyses AL
Cell Adhesion & Migration
Manuscript Link
Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells
Nawara TJ, Yuan J, Seeley LD, Stzul E, and Mattheyses AL
Biophysical Journal
Manuscript Link
2023
Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics
Ankenbauer KE, Rao TC, Mattheyses AL, and Bellis SL
Journal of Biological Chemistry
DrSTAR: Tracking real-time nanometer axial changes
Nawara TN, Dean WF, and Mattheyses AL
Biophysical Journal
Imaging nanoscale axial dynamics at the basal plasma membrane
Nawara TN and Mattheyses AL
The International Journal of Biochemistry and Cell Biology
2022
Defining domain-specific orientational order in the desmosomal cadherins
Dean WF and Mattheyses AL
Biophysical Journal
ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion
Rao TC, Beggs RR, Ankenbauer KE, Hwang J, Ma VP, Salaita K, Bellis SL, and Mattheyses AL
Journal of Biological Chemistry
Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis
Tomasz J Nawara TJ, Williams 2nd YD, Rao TC, Hu Y, Sztul E, Salaita K, and Mattheyses AL
Nature Communications
Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
Rao TC and Mattheyses AL
Journal of Visual Experiments
Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics
Rao TC, Nawara TJ, and Mattheyses AL
Methods in Molecular Biology
Desmosomes undergo dynamic architectural changes during assembly and maturation
Beggs RB. Rao TC, Dean WF, Kowalczyk AP, and Mattheyses AL
Tissue Barriers
2021
Turn-key super-resolution mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
Blanchard AT, Combs JD, Brockman JM, Kellner A, Glazier R, Su H, Bender R, Bazrafshan A, Chen W, Quach ME, Li R, Mattheyses AL, and Salaita K
Nature Communications
Manuscript Link
dSTORM Imaging and Analysis of Desmosome Architecture
Beggs RR, Dean WF, and Mattheyses AL
Methods in Molecular Biology
Chapter Link
2020
Live-cell super-resolved PAINT imaging of piconewton cellular traction forces
Brockman JM, Su H, Blanchard AT, Duan Y, Meyer T, Quach ME, Glazier R, Bazrafshan A, Bender RL, Kellner AV, Ogasawara H, Ma R, Schueder F, Petrich BG, Jungmann R, Li R, Mattheyses AL, Ke Y, and Salaita K
Nature Methods
EGFR activation attenuates the mechanical threshold for integrin tension and focal adhesion formation
Rao TC, Ma VP-Y, Blanchard A, Urner TM, Grandhi S, Salaita K, and Mattheyses AL
Journal of Cell Science
Protein exchange regulates calcium independence of epithelial junctions
Bartle EI, Rao TC, Beggs RR, Dean WF, Urner TM, Kowalczyk AP, and Mattheyses AL
Journal of Cell Biology
Variable Incidence Angle Linear Dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles
Blanchard AT, Brockman JM, Salaita K, and Mattheyses AL
Optics Express
2019
DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates
Glazier R, Brockman JM, Bartle E, Mattheyses AL, Destaing O, and Salaita K
Nature Communications
Pharmacologic inhibition of LIMK1 provides dendritic spine resilience against β-amyloid
Henderson BW, Greathouse KM, Ramdas R, Walker CK, Rao TC, Bach SV, Curtis KA, Day JJ, Mattheyses AL, and Herskowitz JH
Science Signaling
The desmosome is a mesoscale lipid raft-like membrane domain
Lewis JD, Caldara AL, Zimmer SE, Stahley SN, Seybold A, Strong NL, Frangakis AS, Levental I, Wahl JK 3rd, Mattheyses AL, Sasaki T, Nakabayashi K, Hata K, Matsubara Y, Ishida-Yamamoto A, Amagai M, Kubo A, and Kowalczyk AP
Molecular Biology of the Cell
2018
Bridging the gap: Super-resolution microscopy of epithelial cell junctions
Bartle EI, Rao T, Urner TM, and Mattheyses AL
Tissue Barriers
Cutting Edge: Intracellular IFN-β and Distinct Type I IFN Expression Patterns in Circulating Systemic Lupus Erythematosus B Cells
Hamilton JA, Wu Q, Yang P, Luo B, Liu S, Li J, Hong H, Fish EN, Mattheyses AL, Sanz I, Chatham WW, Hsu H, and Mountz JD
Journal of Immunology
Mapping the 3D Orientation of Piconewton Integrin Traction Forces
Brockman JM, Blanchard AT, Ma VP-Y, Derricotte WD, Zhang Y, Fay ME, Lam WA, Evangelista FA, Mattheyses AL*, Salaita K*
Nature Methods
2017
Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization Microscopy
Bartle EI, Urner TM, Raju SS, and Mattheyses AL
Biophysical Journal
High-resolution imaging of muscle attachment structures in Caenorhabditis elegans
Qadota H, Matsunaga Y, Nguyen KCQ, Mattheyses A, Hall DH, and Benian GM
Cytoskeleton
2016
Molecular Organization of the Desmosome as Revealed by Direct Stochastic Optical Reconstruction Microscopy
Stahely SN, Bartle EI, Atkinson CA, Kowalczyk AP, and Mattheyses AL
Journal of Cell Science
”In this issue” featured article
F1000 PRIME Feature
Regulation of claudin/zonula occludens-1 complexes by hetero-claudin interactions
Schlingmann B, Overgaard CE, Molina SA, Lynn KS, Mitchell LA, Dorsainvil White S, Mattheyses AL, Guidot DM, Capaldo CT, and Koval M
Nature Communications
The Sodium Chloride Cotransporter (NCC) and Epithelial Sodium Channel (ENaC) Associate
Mistry AC, Wynne BM, Yu L, Tomilin V, Yue Q, Zhou Y, Al-Khalili O, Mallick R, Cai H, Alli AA, Ko B, Mattheyses A, Bao HF, Pochynyuk O, Theilig F, Eaton DC, Hoover RS
Biochemical Journal
ROCK1 and ROCK2 Inhibition Alters Dendritic Spine Morphology in Hippocampal Neurons
Swanger SA, Mattheyses AL, Gentry EG, and Herskowitz JH
Cellular Logistics
Nanoscale Optomechanical Actuators for Controlling Mechanotransduction in Living Cells
Liu Z, Liu Y, Chang Y, Seyf HR, Henry A, Mattheyses AL, Yehl K, Zhang Y, Huang Z, and Salaita KS
Nature Methods
Super-Resolution Microscopy Reveals Altered Desmosomal Protein Organization in Pemphigus Vulgaris Patient Tissue
Stahley SN, Warren MF, Feldman RJ, Swerlick RA, Mattheyses AL, and Kowalczyk AP
Journal of Investigative Dermatology
2015
Real-time Fluorescence Imaging with 20 nm Axial Resolution
Stabley D, Oh T, Simon SM, Mattheyses AL*, and Salaita KS*
Nature Communications
Foster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions
Mattheyses AL* and Marcus AI
Protein-Protein Interactions: Methods and Applications Second Edition
2014
The ARL2 GTPase is Required for Mitochondrial Morphology, Motility, and Maintenance of ATP Levels
Newman LE, Zhou C, Mudigonda S, Mattheyses AL, Paradies E, Marobbio CMT, and Kahn RA
Plos One
Polarization-Controlled TIRFM With Focal Drift and Spatial Field Intensity Correction
Johnson DS, Toledo-Crow R, Mattheyses AL, and Simon SM
Biophysical Journal
Desmosome assembly and disassembly are membrane raft-dependent
Stahley SN, Saito M, Faundez V, Koval M, Mattheyses AL, and Kowalczyk AP
Plos One
2013
Pharmacologic inhibition of ROCK2 suppresses amyloid-beta production in an Alzheimer disease mouse model
Herskowitz JH, Feng Y, Mattheyses AL, Hales C, Higginbotham L, Duong D, Montine T, Troncoso JC, Thambisetty M, Seyfried N, Levey AI, and Lah JJ
Journal of Neuroscience
The Chlamydomonas mutant pf27 is defective in radial spoke transport in the cilium
Alford LM, Mattheyses AL, Hunter EL, Lin H, Dutcher SK, and Sale WS
Cytoskeleton
Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis
Zlatic SA, Grossniklaus EJ, Ryder PV, Salazar G, Mattheyses AL, Peden AA, and Faundez V
Molecular Biology of the Cell
Conserved Spatial Organization of FG Domains in the Nuclear Pore Complex
Atkinson CE, Mattheyses AL, Kampmann M, and Simon SM
Biophysical Journal
Manuscript Link
Biophysical Journal New and Notable Link
2012
Signaling Network Crosstalk in Human Pluripotent Cells: a Smad2/3-Regulated Switch that Controls the Balance Between Self-Renewal and Differentiation
Singh AM, Reynolds D, Cliff T, Ohtsuka S, Mattheyses AL, Sun Y, Menendez L, Kulik M, and Dalton S
Cell Stem Cell
Domain Topology of Nucleoporin Nup98 Within the Nuclear Pore Complex
Chatel G, Desai SH, Mattheyses AL, Powers MA, and Fahrenkrog B
Journal of Structural Biology